Journal: bioRxiv

Article Title: Elaborating the connections of a closed-loop forebrain circuit in the rat: Circumscribed evidence for novel topography within a cortico-striato-pallidal triple descending projection, with thalamic feedback, to the anterior lateral hypothalamic area

doi: 10.1101/2025.01.18.633747

Figure Lengend Snippet: Histological localization of co-injected anterograde and retrograde tracers. Photomicrographs showing immunodetected PHAL ( left column ), CTB ( right column ), and their corresponding Nissl-stained sections ( middle column ). ( Top row ) Representative injection site in ACBdm at approximately +1.5 mm from bregma. ( Middle row ) Representative injection site in PVTa at approximately −1.8 mm from bregma. ( Bottom row ) Representative injection site in LHAa at approximately −1.8 mm from bregma. Superimposed boundaries are based on the cytoarchitecture shown in the Nissl-stained sections. See Abbreviations for nomenclature. Scale bars: 500 µm.

Article Snippet: Glass micropipettes (tip diamaters: 12–20 µm) were filled with a cocktail containing 2.5% PHAL (Vector Laboratories, Inc., Newark, California, USA; catalog #L-1110) and 0.25% CTB (List Biological Labs, Inc., Campbell, California, USA; catalog #104) dissolved in 10 mM sodium phosphate solution.

Techniques: Injection, Staining

Journal: bioRxiv

Article Title: Elaborating the connections of a closed-loop forebrain circuit in the rat: Circumscribed evidence for novel topography within a cortico-striato-pallidal triple descending projection, with thalamic feedback, to the anterior lateral hypothalamic area

doi: 10.1101/2025.01.18.633747

Figure Lengend Snippet: Convergent PVTa and LHAa connections in ACBdm. (A) Diagrammatic representation of experiments. (B) Darkfield photomicrograph showing PHAL-labeled axons in ACBdm following an injection into PVTa. (C) Brightfield photomicrograph showing CTB-labeled neurons in ACBdm following an injection into LHAa. Boundaries shown in (B) and (C) were determined using adjacent Nissl-stained sections. (D) Mapped distributions of PVTa axons ( green ) and LHAa-projecting neurons ( orange ) across the anterior-posterior span of ACB. The caudoputamen and ACB are indicated using solid boundaries and gray shading. Values in the middle denote approximate distance from bregma. See Abbreviations for nomenclature. Scale bars: 500 µm (B, C) , 1 mm (D) .

Article Snippet: Glass micropipettes (tip diamaters: 12–20 µm) were filled with a cocktail containing 2.5% PHAL (Vector Laboratories, Inc., Newark, California, USA; catalog #L-1110) and 0.25% CTB (List Biological Labs, Inc., Campbell, California, USA; catalog #104) dissolved in 10 mM sodium phosphate solution.

Techniques: Labeling, Injection, Staining

Journal: bioRxiv

Article Title: Elaborating the connections of a closed-loop forebrain circuit in the rat: Circumscribed evidence for novel topography within a cortico-striato-pallidal triple descending projection, with thalamic feedback, to the anterior lateral hypothalamic area

doi: 10.1101/2025.01.18.633747

Figure Lengend Snippet: Convergent ACBdm, LHAa, and PVTa connections in the cytoarchitectonically defined cingulate region (CNG) [more loosely identified as the medial prefrontal cortex]. (A) Diagrammatic representation of experiments. Circles show injected regions and arrows show the direction of the connections. (B) Photomicrographs showing immunodetected PHAL and CTB in CNG at approximately +3.0 mm from bregma. Injection site locations are indicated with abbreviations in the upper-left corners and tracers are indicated in the upper-left corners of each image. Arrowheads point to boundary locations between cortical areas. Boundaries were determined using adjacent Nissl-stained sections. (C) Mapped distributions of PVTa axons ( green ), ACBdm-( purple ) and LHAa-projecting ( orange ) neurons in the CNG. Note the overlap of each label in a subgenual part of the CNG. See Abbreviations for nomenclature. Scale bar: 500 µm (B) .

Article Snippet: Glass micropipettes (tip diamaters: 12–20 µm) were filled with a cocktail containing 2.5% PHAL (Vector Laboratories, Inc., Newark, California, USA; catalog #L-1110) and 0.25% CTB (List Biological Labs, Inc., Campbell, California, USA; catalog #104) dissolved in 10 mM sodium phosphate solution.

Techniques: Injection, Staining

Journal: bioRxiv

Article Title: Elaborating the connections of a closed-loop forebrain circuit in the rat: Circumscribed evidence for novel topography within a cortico-striato-pallidal triple descending projection, with thalamic feedback, to the anterior lateral hypothalamic area

doi: 10.1101/2025.01.18.633747

Figure Lengend Snippet: Convergent ACBdm, LHAa, and PVTa connections in amygdala. (A) Diagrammatic representation of experiments. Circles show injected regions and arrows show the direction of the connections. (B) Photomicrographs showing immunodetected PHAL and CTB in amygdala at approximately −3.5 mm from bregma. Injection site locations are indicated with abbreviations in the upper-left corners and tracers are indicated in the upper-left corners of each image. Boundaries were determined using adjacent Nissl-stained sections. (C) Mapped distributions of PVTa axons ( green ), ACBdm-( purple ) and LHAa-projecting ( orange ) neurons in amygdala. Note the overlap of each label in a medial space between BLAp and BMAp. Values in lower-left corners indicate the approximate distance from bregma and corresponding atlas levels from are indicated in the upper-left corners of each panel. See Abbreviations for nomenclature. Scale bars: 500 µm (B) .

Article Snippet: Glass micropipettes (tip diamaters: 12–20 µm) were filled with a cocktail containing 2.5% PHAL (Vector Laboratories, Inc., Newark, California, USA; catalog #L-1110) and 0.25% CTB (List Biological Labs, Inc., Campbell, California, USA; catalog #104) dissolved in 10 mM sodium phosphate solution.

Techniques: Injection, Staining

Journal: bioRxiv

Article Title: Elaborating the connections of a closed-loop forebrain circuit in the rat: Circumscribed evidence for novel topography within a cortico-striato-pallidal triple descending projection, with thalamic feedback, to the anterior lateral hypothalamic area

doi: 10.1101/2025.01.18.633747

Figure Lengend Snippet: Convergent ACBdm, LHAa, and PVTa connections in hippocampus. (A) Diagrammatic representation of experiments. Circles show injected regions and arrows show the direction of the connections. (B) Photomicrographs showing immunodetected PHAL and CTB in SUBv at approximately −6.0 mm from bregma. Injection site locations are indicated with abbreviations in the upper-left corners and tracers are indicated in the upper-left corners of each image. Note the overlap of each label in a lateral aspect of the anterior SUBv. (C) Mapped distributions of PVTa axons ( green ), ACBdm-( purple ) and LHAa-projecting ( orange ) neurons in hippocampus. Values in lower-left corners indicate the approximate distance from bregma and corresponding atlas levels from are indicated in the upper-left corners of each panel. See Abbreviations for nomenclature. Scale bars: 500 µm (B) .

Article Snippet: Glass micropipettes (tip diamaters: 12–20 µm) were filled with a cocktail containing 2.5% PHAL (Vector Laboratories, Inc., Newark, California, USA; catalog #L-1110) and 0.25% CTB (List Biological Labs, Inc., Campbell, California, USA; catalog #104) dissolved in 10 mM sodium phosphate solution.

Techniques: Injection